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rabbit polyclonal anti total akt  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anti total akt
    Rabbit Polyclonal Anti Total Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+total+akt+antibodies/pmc12319253-4-0-4
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti total akt - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Phospho-proteomics:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Western Blot:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Expressing:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Activity Assay:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Control:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Cell Migration Assay:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Immunofluorescence:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Staining:

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
    Article Snippet: Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-b1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal antiPhospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).

    Article Title: Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II
    Article Snippet: Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).Human recombinant TGF-β1 was purchased from Peprotech (Rocky Hill, NJ, USA).. Rabbit polyclonal anti-Phospho-SMAD2, rabbit polyclonal anti-total SMAD2, mouse monoclonal anti-Phospho p44/42, rabbit polyclonal anti p44/p42, rabbit polyclonal anti-Phospho-Akt, and rabbit polyclonal anti-total Akt antibodies were purchased from Cell Signalling technology, Inc. (Beverly, MA, USA).. Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).Mouse monoclonal anti-human FAK (pY397) antibody was purchased from BD Transduction Laboratories (Franklin Lakes, NJ USA).



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    Fig. 7 Stimulation with TGFb1 increases the expression of JUNB in epithelial HCC cells and promotes the rate of JUNB-mediated transformation into CD90-positive cells. A, B mRNA and protein levels of JUNB and SOX4, induced in a concentration-dependent manner, 24 h after the addition of TGFb1 to the epithelial HCC cell lines Huh7, Huh1, and patient HCC. C Activation of <t>total</t> <t>AKT/pAKT</t> (Ser473), total SMAD3/pSMAD3 (Ser423/425), and total JNK/pJNK (Thr183/Tyl185), 24 h after the addition of 2.5 ng and 5 ng of TGFb1 recombinant protein to the epithelial HCC cell lines Huh7, Huh1, and patient HCC overexpressing JUNB. In A, data are presented as the mean (SD) (n = 3) and were analyzed using the one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.
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    Fig. 7 Stimulation with TGFb1 increases the expression of JUNB in epithelial HCC cells and promotes the rate of JUNB-mediated transformation into CD90-positive cells. A, B mRNA and protein levels of JUNB and SOX4, induced in a concentration-dependent manner, 24 h after the addition of TGFb1 to the epithelial HCC cell lines Huh7, Huh1, and patient HCC. C Activation of <t>total</t> <t>AKT/pAKT</t> (Ser473), total SMAD3/pSMAD3 (Ser423/425), and total JNK/pJNK (Thr183/Tyl185), 24 h after the addition of 2.5 ng and 5 ng of TGFb1 recombinant protein to the epithelial HCC cell lines Huh7, Huh1, and patient HCC overexpressing JUNB. In A, data are presented as the mean (SD) (n = 3) and were analyzed using the one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.
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    Image Search Results


    Fig. 7 Stimulation with TGFb1 increases the expression of JUNB in epithelial HCC cells and promotes the rate of JUNB-mediated transformation into CD90-positive cells. A, B mRNA and protein levels of JUNB and SOX4, induced in a concentration-dependent manner, 24 h after the addition of TGFb1 to the epithelial HCC cell lines Huh7, Huh1, and patient HCC. C Activation of total AKT/pAKT (Ser473), total SMAD3/pSMAD3 (Ser423/425), and total JNK/pJNK (Thr183/Tyl185), 24 h after the addition of 2.5 ng and 5 ng of TGFb1 recombinant protein to the epithelial HCC cell lines Huh7, Huh1, and patient HCC overexpressing JUNB. In A, data are presented as the mean (SD) (n = 3) and were analyzed using the one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Cell death & disease

    Article Title: Transcription factor JUNB is required for transformation of EpCAM-positive hepatocellular carcinoma (HCC) cells into CD90-positive HCC cells in vitro.

    doi: 10.1038/s41419-025-07602-3

    Figure Lengend Snippet: Fig. 7 Stimulation with TGFb1 increases the expression of JUNB in epithelial HCC cells and promotes the rate of JUNB-mediated transformation into CD90-positive cells. A, B mRNA and protein levels of JUNB and SOX4, induced in a concentration-dependent manner, 24 h after the addition of TGFb1 to the epithelial HCC cell lines Huh7, Huh1, and patient HCC. C Activation of total AKT/pAKT (Ser473), total SMAD3/pSMAD3 (Ser423/425), and total JNK/pJNK (Thr183/Tyl185), 24 h after the addition of 2.5 ng and 5 ng of TGFb1 recombinant protein to the epithelial HCC cell lines Huh7, Huh1, and patient HCC overexpressing JUNB. In A, data are presented as the mean (SD) (n = 3) and were analyzed using the one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: The primary antibodies used were anti-JUNB rabbit monoclonal antibody (#3753S, Cell Signaling Technology), anti-total AKT rabbit polyclonal antibody (#9272S), anti-pAKT (Ser473) rabbit monoclonal antibody (#4060S), anti-total SMAD3 rabbit polyclonal antibody (#9513S), anti-pSMAD3 (Ser423/425) rabbit polyclonal antibody (#9520S), anti-total SAPK/JNK rabbit polyclonal antibody (#9252S), anti-pSA’K/JNK (Thr183/Tyr185) rabbit polyclonal antibody (#9251S), anti-SOX4 mouse monoclonal antibody (sc-518016, SantaCruz), and anti-GAPDH mouse monoclonal antibody (sc-32233, SantaCruz).

    Techniques: Expressing, Transformation Assay, Concentration Assay, Activation Assay, Recombinant